Incubation:Article Title: Impaired hydrogen sulfide biosynthesis underlies eccentric contraction–induced force loss in dystrophin-deficient skeletal muscle
Article Snippet: .. The following primary antibodies were incubated with the membranes overnight at 4°C: rabbit polyclonal anti-AE1 (Proteintech, 28131-1-AP; 1:500), rabbit polyclonal anti-ASCT1 (Sigma-Aldrich, AV43827; 1:1,000), rabbit polyclonal anti-ACY1 (Abcam, ab189399; 1:1,000), mouse monoclonal anti-GCL (Thermo Fisher Scientific, MA5-26346; 1:1,000), rabbit polyclonal anti-GSS (Thermo Fisher Scientific, PA5-97810; 1:1,000), rabbit polyclonal anti-GR (Thermo Fisher Scientific, PA570004; 1:1,000), rabbit polyclonal anti-GPX (Thermo Fisher Scientific, PA5-26323; 1:1,000), rabbit polyclonal anti-GRX (Abcam, ab45953; 1:1,000), rabbit polyclonal anti-GSTm1 (Sigma-Aldrich, AV41769; 1:1,000), rabbit polyclonal anti-GSTm2 (Thermo Fisher Scientific, PA5-89264; 1:1,000), rabbit polyclonal anti-CBS (Proteintech, 14787-1-AP; 1:1,000), rabbit polyclonal anti-CSE (Abcam, ab151769; 1:500), mouse monoclonal anti-GOT1 (Abcam, ab239487; 1:1,000), rabbit polyclonal anti-MPST (Novus Biologicals, NBP1-54734; 1:1,000), rabbit monoclonal anti-TRX (Abcam, ab273877; 1:1,000), mouse monoclonal anti-GAPDH (Sigma-Aldrich, G8795; 1:10,000), and rabbit polyclonal anti-GAPDH (Sigma-Aldrich, G9545; 1:10,000). .. Membranes were washed with 0.1% Tween in PBS and incubated in secondary antibodies (DyLight 680 Goat Anti-Mouse IgG [Cell Signaling Technology, 5470S], DyLight 680 Goat Anti-Rabbit IgG [Cell Signaling Technology, 5366S], DyLight 800 Goat Anti-Mouse IgG [Cell Signaling Technology, 5257S], and DyLight 800 Goat Anti-Rabbit IgG [Cell Signaling Technology, 5151S]) for 1 hour at room temperature.
Article Title: Impaired hydrogen sulfide biosynthesis underlies eccentric contraction-induced force loss in dystrophin-deficient skeletal muscle
Article Snippet: .. The following primary antibodies 501 were incubated with the membranes overnight at 4°C: rabbit polyclonal anti-AE1 (Proteintech, 502 28131-1-AP, 1:500), rabbit polyclonal anti-ASCT1 (Sigma, AV43827, 1:1000), rabbit polyclonal 503 anti-ACY1 (Abcam, ab189399, 1:1000), mouse monoclonal anti-GCL (ThermoFisher Scientific, 504 MA5-26346, 1:1000), rabbit polyclonal anti-GS (ThermoFisher Scientific, PA5-97810, 1:1000), 505 rabbit polyclonal anti-GR (ThermoFisher Scientific, PA570004, 1:1000), rabbit polyclonal anti-506 GPX (ThermoFisher Scientific, PA5-26323, 1:1000), rabbit polyclonal anti-GRX (abcam, 507 ab45953, 1:1000), rabbit polyclonal anti-GSTm1 (Sigma-Aldrich, AV41769, 1:1000), rabbit 508 polyclonal anti-GSTm2 (ThermoFisher Scientific, PA5-89264, 1:1000), rabbit polyclonal anti-509 CBS (Proteintech, 14787-1-AP, 1:1000), rabbit polyclonal anti-CSE (abcam, ab151769, 1:500), 510 mouse monoclonal anti-GOT1 (abcam, ab239487, 1:1000), rabbit polyclonal anti-MPST (Novus 511 Bio, NBP1-54734, 1:1000), rabbit monoclonal anti-TRX (abcam, ab273877, 1:1000), mouse 512 monoclonal anti-GAPDH (Sigma-Aldrich, G8795, 1:10000), and rabbit polyclonal anti-GAPDH 513 (Sigma-Aldrich, G9545, 1:10000). .. Membranes were washed with 0.1% Tween in PBS and 514 incubated in secondary antibodies [DyLight 680 Goat anti-Mouse IgG (CST, 5470S), DyLight 680 515 Goat anti-Rabbit IgG (CST, 5366S), DyLight 800 Goat anti-Mouse IgG (CST, 5257S), and 516 DyLight 800 Goat anti-Rabbit IgG (CST, 5151S)] for 1 hour at room temperature The membranes 517 were imaged and analyzed with a LI-COR Odyssey imaging system and associated software 518 (Image Studio Lite v5.2).
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